| Seattle Opera | | | |
| Carlo Montanaro | Dirección | | |
| Kevin Newbury | Dirección de escena | | |
| Neil Patel | Diseño de escena | | |
| Jessica Jahn | Diseño de vestuario | | |
| Joyce El-Khoury | Soprano | Maria Stuarda | |
| Mary Elizabeth Williams | Soprano | Elisabetta | 2016 feb 27, mar 02, 05, 09, 12 |
| Keri Alkema | Soprano | Elisabetta | 2016 feb 28 Matiné, mar 11 |
| John Tessier | Tenor | Earl of Leicester | 2016 feb 27, mar 02, 05, 09, 12 |
| Andrew Owens | Tenor | Earl of Leicester | 2016 feb 28 Matiné, mar 11 |
| Weston Hurt | Barítono | Talbot | |
| Michael Todd Simpson | Barítono | Cecil | |
| Renée Rapier | Mezzosoprano | Anna | |